Catalog Number - 85118-05P

The 85118‑05P Cogent RP C18 ABX™ UHPLC Column is engineered for robust analytical separations under extreme mobile phase conditions, delivering stable performance across a wide pH range (2–12) using a specially engineered, bulky end‑capped C18 bonded phase.

Built on high‑purity silica, this column minimizes unwanted interactions, improving peak shape for acidic, basic, and polar analytes—especially those with high or low pKa values.

✅ Ideal as a USP L1 alternative for methods involving strong acids or bases

50 mm Length – Fast Analysis & Method Scouting

• Designed for rapid separations and short run times

• Ideal for method development and screening workflows

• Reduces:

  • Solvent consumption

  • Cycle time per sample

✅ Preferred for fast UHPLC method optimization and throughput‑driven labs

4.6 mm ID – Standard Analytical Platform

• Compatible with standard analytical UHPLC systems

• Ideal for:

  • method development

  • routine QC analysis

• Provides robust, reproducible performance

Typical Flow Rate Range

• ~0.8–1.5 mL/min

✅ Suitable for standard analytical workflows without low‑flow constraints

1.8 µm Particle – Maximum Efficiency & Resolution

• Higher efficiency than 3 µm and 5 µm columns

• Produces:

  • Sharper peaks

  • Improved resolution

  • Higher peak capacity

✅ Ideal for:

• Reducing run time while maintaining resolution

• Improving separation performance in existing methods

⚠️ Higher backpressure expected → UHPLC system required

Wide pH Stability (pH 2–12) – Extreme Method Capability

• Designed for high and low pH mobile phases

• Suitable for:

  • Strongly acidic compounds

  • Strongly basic compounds

• Enables methods not achievable with standard C18 columns

✅ Provides maximum flexibility in method development

Why Choose RP C18 ABX™ Columns

Extreme pH Ruggedness

• Stable at both high and low pH conditions

• Ideal for challenging analytes and aggressive mobile phases

Improved Peak Shape for Acids & Bases

• Specialized end‑capping reduces secondary interactions

• Enhances chromatographic reproducibility

Higher Throughput & Efficiency

• Faster run times reduce solvent usage and cost

• Increases sample throughput and lab productivity

✅ Excellent choice for method development and optimization workflows

System & Sample Considerations

• ⚠️ Requires UHPLC system capable of high pressure operation

• Recommended sample preparation:

  • 👉 Use 0.2 µm syringe filters for sample preparation before injection

✅ Helps:

• Prevent column fouling

• Maintain peak performance and lifetime

Typical Applications

• HPLC and UHPLC analysis

• Acidic and basic compound separations

• Pharmaceutical method development

• Stability‑indicating methods

• Complex mixtures under extreme pH

• QC and validation workflows

Important Usage Notes

• Designed for UHPLC (high‑pressure) systems

• Best suited for extreme pH conditions (pH 2–12)

• Ideal for fast screening and method development

• Monitor system pressure to ensure safe operation